How To Calculate Your Peptide Dosing
Step 1: Check Your Vial Strength
Start by identifying the total amount of peptide in your vial. This is typically labeled on the vial itself.
Example:
5mg vial (contains 5 milligrams of peptide)
10mg vial
20mg vial
This number tells you how much raw peptide is inside the vial before any reconstitution.
💡 Tip: Always double-check the label for peptide amount before proceeding.
STEP 2: How to Reconstitute a Vial
Follow these simple steps to safely prepare your peptide for research use:
Start by withdrawing the required amount of bacteriostatic water using a sterile syringe.
Carefully insert the needle into the peptide vial and slowly inject the water against the inside wall of the vial to avoid foaming.
Transfer & Gently Mix:
Once the water is added, gently swirl the vial in a circular motion until the peptide is fully dissolved.
Draw Up the Mixed Solution:
Using a clean syringe, draw up the required dose from the reconstituted solution.
💡 Tip: Always store the reconstituted vial in a refrigerator (2–8°C) and use within the recommended timeframe for best stability.
STEP 3: Reconstitution Table
This is how much liquid you add to the vial, whether that’s bacteriostatic water or some other solvent — it’s up to you!
Common options include:
1ml
2ml
5ml
Your choice here affects how much peptide is in each unit (ml or IU) of liquid.
For example:
Adding 1ml of water to a 10mg vial means you’ll get 10mg per ml
Adding 2ml to a 10mg vial gives you 5mg per ml
Adding 5ml gives you 2mg per ml
STEP 4: Calculate the Dose
📌 Example 1:
- If you have a 10mg vial and you’ve added 1ml of bacteriostatic water, it is now 10mg/ml.
- To calculate a 2mg dose, you would draw up 0.2ml.
- If you have a 10mg vial and you’ve added 2ml of water, it is now 5mg/ml.
- To calculate a 2mg dose, you would need 0.4ml (because the water doubled and diluted the strength).
Formula:
Peptide Amount (mg) ÷ Volume of Water (ml) = Dosage per ml
Example:
10mg peptide + 2ml water = 5mg per ml
If using a 100IU insulin syringe, 10 IU = 0.1ml = 0.5mg
Watch: How to Reconstitute a Vial
