How To Calculate Your Peptide Dosing

Step 1: Check Your Vial Strength

Start by identifying the total amount of peptide in your vial. This is typically labeled on the vial itself.
Example:

5mg vial (contains 5 milligrams of peptide)
10mg vial
20mg vial

This number tells you how much raw peptide is inside the vial before any reconstitution.

💡 Tip: Always double-check the label for peptide amount before proceeding.

STEP 2: How to Reconstitute a Vial

Follow these simple steps to safely prepare your peptide for research use:

Add Bacteriostatic Water to Peptide Vial:

Start by withdrawing the required amount of bacteriostatic water using a sterile syringe.
Carefully insert the needle into the peptide vial and slowly inject the water against the inside wall of the vial to avoid foaming.

Transfer & Gently Mix:

Once the water is added, gently swirl the vial in a circular motion until the peptide is fully dissolved.

Draw Up the Mixed Solution:

Using a clean syringe, draw up the required dose from the reconstituted solution.

💡 Tip: Always store the reconstituted vial in a refrigerator (2–8°C) and use within the recommended timeframe for best stability.

STEP 3: Reconstitution Table

This is how much liquid you add to the vial, whether that's bacteriostatic water or some other solvent — it's up to you!

Common options include:

Your choice here affects how much peptide is in each unit (ml or IU) of liquid.

For example:
Adding 1ml of water to a 10mg vial means you’ll get 10mg per ml
Adding 2ml to a 10mg vial gives you 5mg per ml
Adding 5ml gives you 2mg per ml

STEP 4: Calculate the Dose

Use the concentration from the chart above to calculate how much to draw for your dose.

📌 Example 1:

If you have a 10mg vial and you've added 1ml of bacteriostatic water, it is now 10mg/ml.
To calculate a 2mg dose, you would draw up 0.2ml.

📌 Example 2:

If you have a 10mg vial and you've added 2ml of water, it is now 5mg/ml.
To calculate a 2mg dose, you would need 0.4ml (because the water doubled and diluted the strength).

Formula:
Peptide Amount (mg) ÷ Volume of Water (ml) = Dosage per ml

Example:
10mg peptide + 2ml water = 5mg per ml
If using a 100IU insulin syringe, 10 IU = 0.1ml = 0.5mg

💡 Tip: Refer to the chart based on the vial strength in milligrams (mg) and the amount of water added to know how strong your solution is per ml. From there, calculate how much to inject based on the mg you want.